0025 Effects of NLS-4 (Lauflumide) and modafinil in a rat model of circadian rhythm and chronic severe fatigue

Abstract Introduction The chronic fatigue syndrome (CFS) is a heterogeneous disorder characterized by easy fatigability, feverishness, diffuse pains, and depression. Many COVID-19 patients expressed long-term effect of coronavirus (long-COVID), with some characteristics typically found in CFS. To examine the efficacy NLS-4 (Lauflumide), comparison positive control, modafinil, which has been shown to improve recovery from CFS human, severe rats. Methods Male 7-8 weeks old Sprague-Dawley rats were subjected procedure for seven consecutive days. Their locomotor activity home cage was then recorded 3 days following end procedure. A Sham group same measurement circadian but without Rats divided into different groups received, before start dark period, three daily administrations NLS-4, modafinil or vehicle (Control groups). Results induced an impairment activity, that decrease motor during period increase light. Accordingly, difference distance travelled between light lower Control than group. This still present after dose-dependently increased phase and, lesser extent, phase. significant at doses 16, 32 64 mg/kg 128 more sustainable modafinil. Conclusion study suggests rhythm reduced similar magnitude (64 mg/kg) (16 mg/kg). Since these results show could human four times used Support (if any) NLS Pharmaceutics